Title

Uptake of Bacteriophage Λ DNA in Escherichia Coli By Electroporation: An Alternative to in Vitro Packaging

Document Type

Article

Publication Date

12-1-1996

Abstract

By using a high field strength DC pulse of 15 kV/cm and a pulse duration of 5 ms for the transfection of E. coli by bacteriophage λ DNA, we obtained efficiencies of 1.1 × 106 (pfu/μg bacteriophage λ DNA). This represents a 100-fold improvement over the traditional CaCl2/heat shock method and is a viable alternative to the more costly in vitro packaging of recombinant bacteriophage λ DNA for the production of cDNA and genomic libraries.

This document is currently not available here.

Share

COinS