Uptake of Bacteriophage Λ DNA in Escherichia Coli By Electroporation: An Alternative to in Vitro Packaging
By using a high field strength DC pulse of 15 kV/cm and a pulse duration of 5 ms for the transfection of E. coli by bacteriophage λ DNA, we obtained efficiencies of 1.1 × 106 (pfu/μg bacteriophage λ DNA). This represents a 100-fold improvement over the traditional CaCl2/heat shock method and is a viable alternative to the more costly in vitro packaging of recombinant bacteriophage λ DNA for the production of cDNA and genomic libraries.
MSU Digital Commons Citation
Gaynor, John and Fox, Martin G., "Uptake of Bacteriophage Λ DNA in Escherichia Coli By Electroporation: An Alternative to in Vitro Packaging" (1996). Department of Biology Faculty Scholarship and Creative Works. 84.