Purification and Properties of the Guanine Nucleotide Exchange Factor (GEF) from Hela Cells and Its Role in the Initiation of Protein Synthesis
A guanine nucleotide exchange factor (GEF)1 1 The abbreviations used are: eIF-2, eukaryotic initiation factor 2; GEF, GDP exchange factor; HCI, heme-controlled translational inhibitor (an eIF-2α kinase); DAI, double-stranded (ds) RNA-activated translational inhibitor (another eIF-2α kinase); Met-tRNAi, eukaryotic initiator methionyl-tRNA; SDS-PAGE, sodium dodecylsulfate-polyacrylamide gel electrophoresis., catalyzing the exchange of GDP bound to initiation factor eIF-2 for GTP, has been isolated from S3 HeLa cells as the eIF-2·GEF complex and extensively purified by procedures originally developed for purification of GEF from rabbit reticulocytes. The HeLa cell factor resembles rabbit reticulocyte eIF-2·GEF in polypeptide composition, catalytic activity, and inactivation by α-phosphorylated eIF-2.
MSU Digital Commons Citation
Mariano, Thomas M.; Siekierka, John; and Ochoa, Severo, "Purification and Properties of the Guanine Nucleotide Exchange Factor (GEF) from Hela Cells and Its Role in the Initiation of Protein Synthesis" (1986). Department of Chemistry and Biochemistry Faculty Scholarship and Creative Works. 27.