Date of Award

8-2017

Document Type

Thesis

Degree Name

Master of Science (MS)

College/School

College of Science and Mathematics

Department/Program

Biology

Thesis Sponsor/Dissertation Chair/Project Chair

Sandra D. Adams

Committee Member

Lee H. Lee

Committee Member

James Campanella

Abstract

Enteroviruses are single stranded RNA viruses responsible for diseases from respiratory illness to hand-foot-and-mouth disease. As a rapidly evolving group, enteroviruses often gain genetic variability which leads to emergence of additional types, one of which is Enterovirus 69 (EV-69). EV-69 has been associated with respiratory illness, possibly causing symptoms similar to those caused by EV-68. To challenge the ability of the virus to infect cells, this study examined the result of infection in lung cell lines A549 and MRC-5 in response to Epigallocatechin gallate-stearate (EGCG-S) treatment of the virus before infection. EGCG-S, a modified green tea polyphenol, was assessed for its efficacy against EV-69 through examination of changes in cell viability, cytopathic effects, cell proliferation, and reactive oxygen species levels. Apoptosis in treated versus untreated virus infected controls was also assessed. The results of these assays indicate that EGCG-S may be a suitable antiviral in synergy with another drug for inhibiting infection with EV-69 in susceptible cells.

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