Date of Award
Master of Science (MS)
College of Science and Mathematics
Thesis Sponsor/Dissertation Chair/Project Chair
Parkinson's disease, Epigallocatechin gallate, Green tea -- Therapeutic use
Despite the fact that Parkinson’s disease (PD) is the second most common neurodegenerative disease, much of its etiology and pathogenesis remains to be discovered. The main pathological feature of PD is the progressive death of specific dopaminergic (DAergic) neurons in the brain. Oxidative stress induced cell death has been hypothesized as one mechanism responsible for PD pathogenesis.
Hydrogen peroxide (H2O2) is a reactive oxygen species (ROS) that has been implicated in PD as a by-product of dopamine (DA) degradation. 1 -methyl-4-phenyl- 1,2,3,6-tetrahydropyridine (MPTP) has been shown to produce a Parkinsonian syndrome via an oxidative stress induced mechanism. For these reasons, H2O2 and MPTP were used in this study to treat DAergic PC 12 cells to produce a model of PD.
We hypothesized that (-)-epigallocatechin 3-O-gallate (EGCG), a principal chemical component of green tea known for a plethora of health promoting bioactive properties, protects PC 12 cells from H2O2- and MPTP-induced stress.
PC 12 cells treated with EGCG showed increased cell count when compared to untreated cells or cells only treated with H2O2 or MPTP. Cell count increased as EGCG concentration increased.
The results of this study demonstrate that EGCG has neuroprotective effects on H2O2 and MPTP stressed PC 12 cells.
Appert, Elizabeth M., "Neuroprotective Effects of (-)-Epigallocatechin 3-O-gallate (EGCG) on l-methyl-4- phenyl-l,2,3,6-tetrahydropyridine (MPTP) and Hydrogen Peroxide (H2O2) Stressed PC 12 Cells" (2014). Theses, Dissertations and Culminating Projects. 347.