Date of Award
Master of Arts (MA)
College of Humanities and Social Sciences
Thesis Sponsor/Dissertation Chair/Project Chair
Patients suffering from Major Depressive Disorder (MDD) have cognitive impairments that modern antidepressants are unable to treat. There is a large body of research linking glutamate receptors to both cognitive functions and MDD. The present study looks at one type of glutamate receptors, the metabotropic glutamate 5 (mGlu 5) receptor, and the effects of its stimulation on a cognitive task on male Long-Evans rats. The following two hypotheses were used: 1) Memory performance in the delayed non-match to odor task will be progressively impaired as the time between the information and retention trial increases, and 2) administering a mGlu5 positive allosteric modulator such as CDPPB will improve performance on the delayed non-match to odor task. First, a time delayed non-match to odor task (DNMTO) was tested to see if it could function as a test of short-term memory. Results indicated that an increase in inter-trial intervals significantly lowered accuracy in the task. This DNMTO task thus showed a more ethologically relevant working memory task than the commonly used visual-based tasks as rats have poor vision. Then the rats were dosed with 3-Cyano-N-(1,3-diphenyl-1H-pyrazol-5-yl)benzamide (CDPPB), a positive allosteric modulator for mGlu 5 receptors, to test for an improvement on the task. No significant differences were found between control or any dose (1, 3, or 10 mg/kg IP) in either accuracy, latency, distance or speed. However, large effect sizes were found which implies that there was not enough power due the low sample sizes caused by attrition. This study was part of a larger research project delving into different glutamate receptors and their impact on both cognitive function and MDD.
Duarte, Stacy Jesus, "The Effect of Metabotropic Glutamate 5 Receptors Stimulation on Delayed Non-Match to Odor Performance in Long-Evans Male Rats" (2020). Theses, Dissertations and Culminating Projects. 475.
Available for download on Thursday, May 12, 2022