Journal / Book Title
International Journal of Molecular Sciences
Induction of heme oxygenase-1 (HO-1) has been demonstrated to decrease body weight and improve insulin sensitivity in several models of obesity in rodents. To further study the role of HO-1 in adipose tissue, we created an adipose-specific HO-1 knockout mouse model. Male and female mice were fed either a control or a high-fat diet for 30 weeks. Body weights were measured weekly and body composition, fasting blood glucose and insulin levels were determined every six weeks. Adipocyte-specific knockout of HO-1 had no significant effect on body weight in mice fed a high-fat diet but increased body weight in female mice fed a normal-fat diet. Although body weights were not different in females fed a high fat diet, loss of HO-1 in adipocytes resulted in significant alterations in body composition. Adipose-specific HO-1 knockout resulted in increased fasting hyperglycemia and insulinemia in female but not male mice on both diets. Adipose-specific knockout of HO-1 resulted in a significant loss of HO activity and a decrease in the protein levels of adiponectin in adipose tissue. These results demonstrate that loss of HO-1 in adipocytes has greater effects on body fat and fasting hyperglycemia in a sex-dependent fashion and that expression of HO-1 in adipose tissue may have a greater protective role in females as compared to males.
MSU Digital Commons Citation
Hosick, Peter A.; Weeks, Mary Frances; Hankins, Michael W.; Moore, Kyle H.; and Stec, David E., "Sex-Dependent Effects of HO-1 Deletion from Adipocytes in Mice" (2017). Department of Exercise Science and Physical Education Scholarship and Creative Works. 86.
Hosick, Peter A., Mary Frances Weeks, Michael W. Hankins, Kyle H. Moore, and David E. Stec. "Sex-dependent effects of HO-1 deletion from adipocytes in mice." International Journal of Molecular Sciences 18, no. 3 (2017): 611.
Clinical Epidemiology Commons, Dietetics and Clinical Nutrition Commons, Epidemiology Commons, Health and Physical Education Commons, Health Services Administration Commons, Health Services Research Commons, Medical Sciences Commons, Nutritional and Metabolic Diseases Commons, Other Medicine and Health Sciences Commons, Patient Safety Commons, Physical Sciences and Mathematics Commons, Public Health Education and Promotion Commons, Rehabilitation and Therapy Commons