Title

Role of E267 in Proton Transfer

Presenter Information

Chrisnaelle Beaucejour

Presentation Type

Poster

Faculty Advisor

Jaclyn Catalano

Access Type

Event

Start Date

26-4-2023 12:30 PM

End Date

26-4-2023 1:30 PM

Description

Cytochrome P450 is a heme protein found in the liver that metabolizes drugs in the body. CYP2S1 is a mammalian CYP that is overexpressed in cancer cells and can be a potential drug target. CYP2S1’s chemistry and mechanism is different from other CYPs and we hypothesize that it is due to the lack of a conserved acid residue. To study the role of the conserved acidic residue we are using a bacterial model (BM-3) because it is easier to express and purify. The wild type of the protein has glutamic (E) acid found at residue 267. The research question we want to answer is whether E267 is involved in proton transfer. Deuterium studies were done to test the catalytic activity of different single mutations at the residue 267. Deuterium has a higher viscosity than water therefore to rule out effects of viscosity, catalytic activity, and protein stability with different percentages of glycerol studies were also performed.

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Apr 26th, 12:30 PM Apr 26th, 1:30 PM

Role of E267 in Proton Transfer

Cytochrome P450 is a heme protein found in the liver that metabolizes drugs in the body. CYP2S1 is a mammalian CYP that is overexpressed in cancer cells and can be a potential drug target. CYP2S1’s chemistry and mechanism is different from other CYPs and we hypothesize that it is due to the lack of a conserved acid residue. To study the role of the conserved acidic residue we are using a bacterial model (BM-3) because it is easier to express and purify. The wild type of the protein has glutamic (E) acid found at residue 267. The research question we want to answer is whether E267 is involved in proton transfer. Deuterium studies were done to test the catalytic activity of different single mutations at the residue 267. Deuterium has a higher viscosity than water therefore to rule out effects of viscosity, catalytic activity, and protein stability with different percentages of glycerol studies were also performed.