Measuring the effects of co-solvents on hydrogen bonding in water through infrared
Presentation Type
Abstract
Faculty Advisor
Johannes Schelvis
Access Type
Event
Start Date
25-4-2025 9:00 AM
End Date
25-4-2025 9:59 AM
Description
Co-solvents, such as ��-glycerophosphate, sucrose, and trehalose, have been found in thermophilic organisms and are thought to help stabilize protein structure at high temperatures. This study analyzes temperature-dependent changes in the water libration-bending mode to determine if the co-solvents stabilize water structure by strengthening hydrogen-bonding interactions between water molecules. Additionally, we investigated the role of these co-solvents in stabilizing photolyase at high temperature to assess their protective effects on protein structure/function by investigating the enzyme's activity. By studying how ��-glycerophosphate, sucrose, and trehalose influence hydrogen bonding in water at different temperatures and their impact on DNA repair through photolyase activity, we hope to gain a better understanding of how the co-solvents are able to impact protein stability at high temperatures.
Measuring the effects of co-solvents on hydrogen bonding in water through infrared
Co-solvents, such as ��-glycerophosphate, sucrose, and trehalose, have been found in thermophilic organisms and are thought to help stabilize protein structure at high temperatures. This study analyzes temperature-dependent changes in the water libration-bending mode to determine if the co-solvents stabilize water structure by strengthening hydrogen-bonding interactions between water molecules. Additionally, we investigated the role of these co-solvents in stabilizing photolyase at high temperature to assess their protective effects on protein structure/function by investigating the enzyme's activity. By studying how ��-glycerophosphate, sucrose, and trehalose influence hydrogen bonding in water at different temperatures and their impact on DNA repair through photolyase activity, we hope to gain a better understanding of how the co-solvents are able to impact protein stability at high temperatures.
Comments
Poster presentation at the 2025 Student Research Symposium.