In-vitro analysis of cobalt sulfate exposure on human fibroblast lung cell health

Presentation Type

Abstract

Faculty Advisor

Ann Dilorenzo

Access Type

Event

Start Date

25-4-2025 10:30 AM

End Date

25-4-2025 11:29 AM

Description

The rapid shift toward sustainable energy solutions, particularly in the transportation sector, has driven a surge in electric vehicle (EV) production. As the demand for EVs continues to rise, so does the global reliance on cobalt mining and its subsequent use in commercial applications despite the long-term health consequences of increased cobalt exposure remaining largely unclear. An in vitro study using cobalt sulfate to induce toxicity was used to investigate the effect this toxin had on cellular proliferation and other cellular processes in a MRC-5 cell line. Preliminary results show that increasing cobalt sulfate toxicity concentrations have a negative impact on cellular morphology. Additionally, a half-maximal inhibitory concentration (IC50) of 0.09 mM CoSO4 was determined and used for future experiments. CoSO4 toxicity has a negative effect on the cell line and future work seeks to understand what cellular processes are being affected and whether or not these cells can be salvaged after toxicity.

Comments

Poster presentation at the 2025 Student Research Symposium.

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Apr 25th, 10:30 AM Apr 25th, 11:29 AM

In-vitro analysis of cobalt sulfate exposure on human fibroblast lung cell health

The rapid shift toward sustainable energy solutions, particularly in the transportation sector, has driven a surge in electric vehicle (EV) production. As the demand for EVs continues to rise, so does the global reliance on cobalt mining and its subsequent use in commercial applications despite the long-term health consequences of increased cobalt exposure remaining largely unclear. An in vitro study using cobalt sulfate to induce toxicity was used to investigate the effect this toxin had on cellular proliferation and other cellular processes in a MRC-5 cell line. Preliminary results show that increasing cobalt sulfate toxicity concentrations have a negative impact on cellular morphology. Additionally, a half-maximal inhibitory concentration (IC50) of 0.09 mM CoSO4 was determined and used for future experiments. CoSO4 toxicity has a negative effect on the cell line and future work seeks to understand what cellular processes are being affected and whether or not these cells can be salvaged after toxicity.