Coxsackievirus and Adenovirus Receptor (CAR) Mediates Trafficking of Acid Sensing Ion Channel 3 (ASIC3) Via PSD-95
Document Type
Article
Publication Date
8-17-2012
Abstract
We have previously shown that the Coxsackievirus and adenovirus receptor (CAR) can interact with post-synaptic density 95 (PSD-95) and localize PSD-95 to cell-cell junctions. We have also shown that activity of the acid sensing ion channel (ASIC3), a H+-gated cation channel that plays a role in mechanosensation and pain signaling, is negatively modulated by PSD-95 through a PDZ-based interaction. We asked whether CAR and ASIC3 simultaneously interact with PSD-95, and if so, whether co-expression of these proteins alters their cellular distribution and localization. Results indicate that CAR and ASIC3 co-immunoprecipitate only when co-expressed with PSD-95. CAR also brings both PSD-95 and ASIC3 to the junctions of heterologous cells. Moreover, CAR rescues PSD-95-mediated inhibition of ASIC3 currents. These data suggest that, in addition to activity as a viral receptor and adhesion molecule, CAR can play a role in trafficking proteins, including ion channels, in a PDZ-based scaffolding complex.
DOI
10.1016/j.bbrc.2012.07.033
Montclair State University Digital Commons Citation
Excoffon, Katherine J.D.A.; Kolawole, Abimbola O.; Kusama, Nobuyoshi; Gansemer, Nicholas D.; Sharma, Priyanka; Hruska-Hageman, Alesia M.; Petroff, Elena; and Benson, Christopher J., "Coxsackievirus and Adenovirus Receptor (CAR) Mediates Trafficking of Acid Sensing Ion Channel 3 (ASIC3) Via PSD-95" (2012). Department of Biology Faculty Scholarship and Creative Works. 382.
https://digitalcommons.montclair.edu/biology-facpubs/382