DNA Metabolism During Infection of Anacystis Nidulans By Cyanophage AS-1: 6. Effect of Hydroxyurea and Nalidixic Acid on the Development of Cyanophage AS-1
Document Type
Article
Publication Date
12-1-1985
Journal / Book Title
Microbios
Abstract
The use of the DNA inhibitors hydroxyurea (HU) and nalidixic acid (NAL) to elucidate patterns of DNA metabolism in AS-1 infected A. nidulans have led to several conclusions. First, HU and NAL at concentrations of 500 and 100 micrograms/ml, respectively, inhibited DNA synthesis in synchronized A. nidulans. Protein and chlorophyll synthesis remained essentially unchanged as did turbidity increases. Second, the complete burst size of the cyanophage AS-1 was severely affected by HU and NAL treatment and burst sizes of 8 and 2.6, respectively, were obtained. Third, inhibitor treatment of infected cultures caused a premature release of phage at 12 h instead of 16 h post infection. Finally, HU treatment of the host cell had no effect on the formation of PIL-DNA, whereas NAL pretreatment blocked its formation.
Journal ISSN / Book ISBN
ISSN 0026-2633
OCLC 1643639
Montclair State University Digital Commons Citation
Lee, Lee; Cohn, G.; Blamire, J.; and McGowan, R., "DNA Metabolism During Infection of Anacystis Nidulans By Cyanophage AS-1: 6. Effect of Hydroxyurea and Nalidixic Acid on the Development of Cyanophage AS-1" (1985). Department of Biology Faculty Scholarship and Creative Works. 415.
https://digitalcommons.montclair.edu/biology-facpubs/415
Published Citation
Hwang Lee, L., Cohn, G., & Blashka, K. H. (1983). DNA metabolism during infection of Anacystis nidulans by cyanophage AS-1: 4. Studies on the source of PIL-DNA. Microbios, 36(144), 101-111.
