Synthesis of Proteins By Chloroplasts from Iron-Deficient Euglena Gracilis
Document Type
Article
Publication Date
10-1-1982
Journal / Book Title
Archives of Biochemistry and Biophysics
Abstract
Chloroplasts isolated from Euglena gracilis made iron deficient by growth on 0.5 μm iron show distinct qualitative and quantitative changes in their polypeptide composition in comparison with iron-sufficient (40 μm) chloroplasts. These changes were noted in the stromal, thylakoid, and envelope subfractions. Iron-deficient chloroplasts have a sedimentation behavior similar to that of iron-sufficient chloroplasts and also contain substantial amounts of ribulose-1,5-bisphosphate carboxylase. In addition, iron-deficient chloroplasts incorporate [3H]leucine into polypeptides at rates about one-third of those from control chloroplasts (40 μm Fe) on a per-microgram-chlorophyll basis. Incorporation of [3H]leucine into specific polypeptides, resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, shows relatively normal synthesis of the large subunit of ribulose-1,5-bisphosphate carboxylase and two of the three major chloroplast-derived polypeptides of the thylakoids. No incorporation was detected, however, into a polypeptide of ca. 33 kd which is synthesized by normal plastids. Iron-deficient chloroplasts also synthesize a stromal polypeptide of ca. 85 kd not seen in chloroplasts from normal cells. This evidence is consistent with a direct or indirect role for iron in the regulation of synthesis of specific proteins in the chloroplast.
DOI
10.1016/0003-9861(82)90349-6
Montclair State University Digital Commons Citation
Gaynor, John, "Synthesis of Proteins By Chloroplasts from Iron-Deficient Euglena Gracilis" (1982). Department of Biology Faculty Scholarship and Creative Works. 94.
https://digitalcommons.montclair.edu/biology-facpubs/94
Published Citation
Gaynor J. J. (1982). Synthesis of proteins by chloroplasts from iron-deficient Euglena gracilis. Archives of biochemistry and biophysics, 218(1), 309–319. https://doi.org/10.1016/0003-9861(82)90349-6
