Histone deacetylase inhibitors and cell proliferation in pea root meristems
Document Type
Article
Publication Date
9-20-2000
Journal / Book Title
Phytochemistry
Abstract
The histone deacetylase (HDA) inhibitors, trichostatin A (TSA) and HC toxin halt mitosis in cultured root meristems of Pisum sativum, while the anti-protozoan HDA inhibitor apicidin is ineffective. Two-dimensional PAGE of proteins from root meristems exposed to TSA and HC toxin did not show significant differences compared to controls, although a previously tested HDA inhibitor, butyrate, exhibited dramatic variations in its protein profile. Northern analysis of butyrate- and TSA-treated root meristems indicated that non-proliferating cells are expressing significant amounts of transcripts of the known cell proliferation associated genes: histone H2A, MAP kinase, cycA2:1 and cdc2. Western analysis reveals the presence of hyperacetylated nuclear proteins in HDA-inhibitor treated cells. These results suggest that the HDA inhibitors, butyrate and TSA, halt mitosis without down-regulating genes that typically have low or nonexistent expression levels in non-dividing cells. (C) 2000 Elsevier Science Ltd.
DOI
10.1016/S0031-9422(00)00195-3
Journal ISSN / Book ISBN
0034692417 (Scopus)
MSU Digital Commons Citation
Murphy, James P.; McAleer, Jody P.; Uglialoro, Anthony; Papile, Jessica; Weniger, Jason; Bethelmie, Floyd; and Tramontano, William A., "Histone deacetylase inhibitors and cell proliferation in pea root meristems" (2000). Bloomfield College of Montclair State University Scholarship and Creative Works. 11.
https://digitalcommons.montclair.edu/bloomfield_facpubs/11
