Initiation of Protein Synthesis in Eukaryotes. Nature of Ternary Complex Dissociation Factor.

Authors

John Siekierka, Montclair State UniversityFollow
Asis Datta, Jawaharlal Nehru University
Ljubica Mauser, RocheFollow
Severo Ochoa, Universidad Autónoma de MadridFollow

Document Type

Article

Publication Date

4-25-1982

Journal / Book Title

Journal of Biological Chemistry

Abstract

Excessive concentrations of the eukaryotic initiation factor 2(eIF-2).stimulating protein, a factor that catalyzes the formation of binary (GTP.eIF-2) and ternary (GTP.eIF-2.initiator methionyl-tRNA) initiation complexes at physiological Mg2+ concentrations, can cause ternary complex dissociation when the Mg2+ concentration is raised from 0.5 to 5.0 mM. Stimulation of ternary complex formation and dissociation have similar (a) pH optima, (b) metal ion specificity, and (c) sensitivity to phosphorylation of the eIF-2 alpha subunit. The results suggest that ternary complex dissociation is an artifact with no physiological significance.

Comments

This article is Open Access and distributed under a Creative Commons 4.0 license.

DOI

10.1016/S0021-9258(18)34700-8

Montclair State University Digital Commons Citation

Siekierka, John; Datta, Asis; Mauser, Ljubica; and Ochoa, Severo, "Initiation of Protein Synthesis in Eukaryotes. Nature of Ternary Complex Dissociation Factor." (1982). Department of Chemistry and Biochemistry Faculty Scholarship and Creative Works. 124.
https://digitalcommons.montclair.edu/chem-biochem-facpubs/124