Radioiodination of Interleukin 2 to High Specific Activities by the Vapor-Phase Chloramine T Method

Document Type

Article

Publication Date

8-1-1988

Abstract

Recombinant human interleukin 2 (IL-2) was radioiodinated utilizing the vapor phase chloramine T method of iodination. The method is rapid, reproducible, and allows the efficient radioiodination of IL-2 to specific activities higher than those previously attained with full retention of biological activity. IL-2 radioiodinated by this method binds with high affinity to receptors present on phytohemagglutinin-stimulated peripheral blood lymphocytes and should be useful for the study of receptor structure and function.

DOI

10.1016/0003-2697(88)90477-0

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