Substrate Binding Modulates the Reduction Potential of DNA Photolyase
Document Type
Article
Publication Date
8-3-2005
Journal / Book Title
Journal of the American Chemical Society
Abstract
The reduction potential of the (FADH-/FADH•) couple in DNA photolyase was measured, and the value was found to be significantly higher than the values estimated in the literature. In the absence of substrate, the enzyme has a reduction potential of 16 ± 6 mV vs NHE. In the presence of excess substrate the reduction potential increases to 81 ± 8 mV vs NHE. The increase in reduction potential has physiological relevance since it gives the catalytic state greater resistance to oxidation. This is the first measurement of a reduction potential for this class of DNA-repair enzymes and the larger family of blue-light photoreceptors.
DOI
10.1021/ja051441r
Montclair State University Digital Commons Citation
Gindt, Yvonne; Schelvis, Johannes; Thoren, Katie L.; and Huang, Tina H., "Substrate Binding Modulates the Reduction Potential of DNA Photolyase" (2005). Department of Chemistry and Biochemistry Faculty Scholarship and Creative Works. 385.
https://digitalcommons.montclair.edu/chem-biochem-facpubs/385
