Single-Molecule Dynamics Associated with Protein Folding and Deformations of Light-Harvesting Complexes
Document Type
Book Chapter
Publication Date
1-1-2001
Journal / Book Title
Single Molecule Spectroscopy
Abstract
Experimental understanding of biological and chemical systems is based primarily on measurements of many molecules and therefore kinetic measurements generally display the evolution of the mean of that ensemble. However, since heterogeneity of structure and mechanism is required to describe complex systems such as proteins and other biological assemblies, this useful paradigm can break down. Recent developments in single molecule fluorescence detection have allowed the study of single biological molecules and single biological assemblies under physiological conditions [1–28]. By following the trajectory of individual members of an equilibrium ensemble as they evolve in time, the fluctuation rates, reaction rate constants, and distributions of other properties can be evaluated.
DOI
10.1007/978-3-642-56544-1_18
Montclair State University Digital Commons Citation
Talaga, David; Jia, Yiwei; Bopp, Martin A.; Sytnik, Alexander; DeGrado, William F.; Cogdell, R. J.; and Hochstrasser, Robin M., "Single-Molecule Dynamics Associated with Protein Folding and Deformations of Light-Harvesting Complexes" (2001). Department of Chemistry and Biochemistry Faculty Scholarship and Creative Works. 570.
https://digitalcommons.montclair.edu/chem-biochem-facpubs/570
