Immediate and Long-Term Impacts of UV-C Irradiation on Photosynthetic Capacity, Survival and Microcystin-LR Release Risk of Microcystis Aeruginosa

Document Type

Article

Publication Date

3-15-2012

Abstract

In this study, the immediate and long-term impacts of shortwave ultraviolet (UV-C) irradiation on photosynthetic capacity, survival, and recovery of Microcystis aeruginosa were investigated. The risk of microcystin-LR (MC-LR) release during irradiation was also estimated. The cell density was determined by a flow cytometry, and typical chlorophyll fluorescence parameters, including the effective quantum yield, photosynthetic efficiency and maximal electron transport rate, were measured by a pulse amplitude modulated (PAM) fluorometer. Under various UV-C dosages (140-4200mJ cm -2 ), photosynthetic capacities were reduced, to different degrees, accompanied by slight cytoclasis and complete degradation of extracellular MC-LR immediately after irradiation. In a 6-d cultivation following UV-C irradiation, cell density and extracellular MC-LR in the samples treated by 140mJcm -2 UV-C irradiation increased from 4.0×10 6 cellsmL -1 and 8μgL -1 to 5.1×10 6 cellsmL -1 and 20μgL -1 , respectively. Significant M. aeruginosa cytoclasis (cell density from 4.0×10 6 to 1.0×10 6 cellsmL -1 ) and MC-LR release (2-25μgL -1 ) occurred when the UV-C dosage reached 350mJcm -2 . Cell cytoclasis and MC-LR release were enhanced in the cultivated samples under higher UV-C dosages. Results revealed that photosynthetic parameters were useful tools to predict the recovery profiles of M. aeruginosa cells, and the MC-LR release risk should be considered after UV-C inactivation.

DOI

10.1016/j.watres.2011.12.025

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