Date of Award

5-2013

Document Type

Thesis

Degree Name

Master of Science (MS)

College/School

College of Science and Mathematics

Department/Program

Biology

Thesis Sponsor/Dissertation Chair/Project Chair

Carlos A. Molina

Committee Member

Elena Petroff

Committee Member

Kirsten Monsen

Abstract

Inducible cAMP early repressor (ICER) has been known to moderate cAMP antiproliferative actions. It has ovarian- specific properties and its gene product has been shown to act as a tumor suppressor. It has been previously shown that ICER has been targeted for expression in the ovaries via a transgenic mouse model to create an increased level of ovulation. In this paper, we cloned the following ovarian-specific promoters: CYP19A1, FSH, ZP3, and GDF9. Plasmid DNA was constructed with the aforementioned ovarian specific promoters using a three-fragment vector construction method and EGFP was used as a marker to follow whether plasmid DNA was successfully incorporated into the ovarian tissue. Upon vector construction, micro injection into zebrafish eggs was performed to produce the transgenic model. Upon transgenesis into the zebrafish model, Danio rerio, we ultimately want to test whether expression will cause an increase in ovulation. If successful overexpression of these promoters in the ovaries of Danio rerio causes an increase in ovulation, this can have significant implications for future research for increasing the production of livestock or fish.

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