Generation of a Transgenic Zebrafish (Danio rerio) Expressing the Transcriptional Repressor Inducible cAMP Early Repressor (ICER)
Date of Award
Master of Science (MS)
College of Science and Mathematics
Thesis Sponsor/Dissertation Chair/Project Chair
Carlos A. Molina
Inducible cAMP early repressor (ICER) has been known to moderate cAMP antiproliferative actions. It has ovarian- specific properties and its gene product has been shown to act as a tumor suppressor. It has been previously shown that ICER has been targeted for expression in the ovaries via a transgenic mouse model to create an increased level of ovulation. In this paper, we cloned the following ovarian-specific promoters: CYP19A1, FSH, ZP3, and GDF9. Plasmid DNA was constructed with the aforementioned ovarian specific promoters using a three-fragment vector construction method and EGFP was used as a marker to follow whether plasmid DNA was successfully incorporated into the ovarian tissue. Upon vector construction, micro injection into zebrafish eggs was performed to produce the transgenic model. Upon transgenesis into the zebrafish model, Danio rerio, we ultimately want to test whether expression will cause an increase in ovulation. If successful overexpression of these promoters in the ovaries of Danio rerio causes an increase in ovulation, this can have significant implications for future research for increasing the production of livestock or fish.
Tsatsos, Theodora, "Generation of a Transgenic Zebrafish (Danio rerio) Expressing the Transcriptional Repressor Inducible cAMP Early Repressor (ICER)" (2013). Theses, Dissertations and Culminating Projects. 1073.