Caenorhabditis Elegans Paraoxonase-Like Proteins Control the Functional Expression of DEG/ENaC Mechanosensory Proteins
Document Type
Article
Publication Date
4-15-2016
Journal / Book Title
Molecular biology of the cell
Abstract
Caenorhabditis elegans senses gentle touch via a mechanotransduction channel formed from the DEG/ENaC proteins MEC-4 and MEC-10. An additional protein, the paraoxonase-like protein MEC-6, is essential for transduction, and previous work suggested that MEC-6 was part of the transduction complex. We found that MEC-6 and a similar protein, POML-1, reside primarily in the endoplasmic reticulum and do not colocalize with MEC-4 on the plasma membrane in vivo. As with MEC-6, POML-1 is needed for touch sensitivity, the neurodegeneration caused by the mec-4(d) mutation, and the expression and distribution of MEC-4 in vivo. Both proteins are likely needed for the proper folding or assembly of MEC-4 channels in vivo as measured by FRET. MEC-6 detectably increases the rate of MEC-4 accumulation on the Xenopus oocyte plasma membrane. These results suggest that MEC-6 and POML-1 interact with MEC-4 to facilitate expression and localization of MEC-4 on the cell surface. Thus MEC-6 and POML-1 act more like chaperones for MEC-4 than channel components.
DOI
10.1091/mbc.E15-08-0561
Montclair State University Digital Commons Citation
Chen, Yushu; Bharill, Shashank; Altun, Zeynep; O'Hagan, Robert; Coblitz, Brian; Isacoff, Ehud Y.; and Chalfie, Martin, "Caenorhabditis Elegans Paraoxonase-Like Proteins Control the Functional Expression of DEG/ENaC Mechanosensory Proteins" (2016). Department of Biology Faculty Scholarship and Creative Works. 85.
https://digitalcommons.montclair.edu/biology-facpubs/85
Published Citation
Chen, Y., Bharill, S., Altun, Z., O'Hagan, R., Coblitz, B., Isacoff, E. Y., & Chalfie, M. (2016). Caenorhabditis elegans paraoxonase-like proteins control the functional expression of DEG/ENaC mechanosensory proteins. Molecular biology of the cell, 27(8), 1272–1285. https://doi.org/10.1091/mbc.E15-08-0561
Comments
This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).