Title
Comparing CYP2S1 and CYP102A1: Factors that influence the chemistry of CYP2S1
Presentation Type
Event
Start Date
27-4-2019 8:45 AM
End Date
27-4-2019 9:24 AM
Abstract
Cytochromes P450s have been investigated as drug targets for anticancer prodrugs (to be activated by P450 only in cancer cells). Current investigation involves mutating a well-studied P450 BM-3 to mimic the chemistry of CYP2S1. We tested different structured substrates and saw the enzymatic activity is affected when an acidic residue in the oxygen binding pocket is mutated to a methionine.
Comparing CYP2S1 and CYP102A1: Factors that influence the chemistry of CYP2S1
Cytochromes P450s have been investigated as drug targets for anticancer prodrugs (to be activated by P450 only in cancer cells). Current investigation involves mutating a well-studied P450 BM-3 to mimic the chemistry of CYP2S1. We tested different structured substrates and saw the enzymatic activity is affected when an acidic residue in the oxygen binding pocket is mutated to a methionine.