MiniBagel and Mooshroom: Two Novel Arthrobacter phage
Presentation Type
Poster
Faculty Advisor
Kristen Monsen
Access Type
Event
Start Date
26-4-2024 9:45 AM
End Date
26-4-2024 10:44 AM
Description
Bacteriophage (phage) are viruses that infect bacterial hosts. As part of the SEA-PHAGES program at MSU, students isolate, characterize, and annotate the genome of a previously undiscovered phage. Phage were obtained from soil samples collected throughout New Jersey and isolated using Mycobacterium smegmatis and Arthrobacter sp. as hosts. To date, students have isolated 283 phage using Mycobacterium smegmatis, and Arthrobacter sp. as hosts. Of these 283 phage, 28 have been sequenced and 22 have been deposited in GenBank (with three currently in review). The genomic DNA of two novel Arthrobacter sp. phage, Mooshroom and MiniBagel, were sent to a commercial sequencing facility for complete genome sequencing. Bioinformatics software programs including DNA Master, Phamerator, and HHPred were used to identify the most likely start codons for all coding genes in each genome. Possible gene functions were also determined for some genes. MiniBagel is a cluster AM phage with approximately 100 coding genes, and Mooshroom is a cluster AU phage with approximately 96 coding genes. Surprisingly, there were approximately 15 genes in each genome that were not called by DNA Master in the initial auto-annotation and had to be added manually. Pairwise alignments between genomes shows a 44.47% identity between MiniBagel and Mooshroom. Work on these annotations is ongoing and the students at MSU anticipate their annotations to be complete by May 1, 2023.
MiniBagel and Mooshroom: Two Novel Arthrobacter phage
Bacteriophage (phage) are viruses that infect bacterial hosts. As part of the SEA-PHAGES program at MSU, students isolate, characterize, and annotate the genome of a previously undiscovered phage. Phage were obtained from soil samples collected throughout New Jersey and isolated using Mycobacterium smegmatis and Arthrobacter sp. as hosts. To date, students have isolated 283 phage using Mycobacterium smegmatis, and Arthrobacter sp. as hosts. Of these 283 phage, 28 have been sequenced and 22 have been deposited in GenBank (with three currently in review). The genomic DNA of two novel Arthrobacter sp. phage, Mooshroom and MiniBagel, were sent to a commercial sequencing facility for complete genome sequencing. Bioinformatics software programs including DNA Master, Phamerator, and HHPred were used to identify the most likely start codons for all coding genes in each genome. Possible gene functions were also determined for some genes. MiniBagel is a cluster AM phage with approximately 100 coding genes, and Mooshroom is a cluster AU phage with approximately 96 coding genes. Surprisingly, there were approximately 15 genes in each genome that were not called by DNA Master in the initial auto-annotation and had to be added manually. Pairwise alignments between genomes shows a 44.47% identity between MiniBagel and Mooshroom. Work on these annotations is ongoing and the students at MSU anticipate their annotations to be complete by May 1, 2023.
Comments
Additional Authors: Amina Abada, Noor Abdelal, Tanya Abu Kamal, Wilmary Acosta, Sandra D. Adams, Olivia Barnabei, Himani Batham, Alissa, Jayla Brown, Mikeily Campos, Tiziana Castillo, Vicente Contreras, Luca Contursi, Jessica Davrayev, Nory Derius, Alexander Diaz, Liyah Evans, Alexander Fenton, Ethan Ferrer, James Galapate, Brayan Gutierrez, Stephannie Gutierrez, Kristen Guzman, Reem Hajjar, Zarifa Hamdan, Christian Huilotl, Julia Iwinski, Yara Jamhour, Deborah Jean Louis, Alexander Kuzmich, Angel Mejia, Litzy Mejia, Gihany Melendez, Esteban Ocana, Sara Orzechowski, Jhorleny Padilla-Garcia, Jose Perez, Fatima Perez-Sosa, Daniela Ramos, Araceli Rivera, Brielle Rodriguez, Madelyn Roque, Nyasia Scott, Amanda Speno, Kayla Swaka, Annmarie Thomas, Isabella Tramontana, Quinn Vega, Ethan Woroniecki, Dunia Zawahreh