Cardiac/Diabetes Story
Presentation Type
Poster
Faculty Advisor
Ann Dilorenzo
Access Type
Event
Start Date
26-4-2024 11:15 AM
End Date
26-4-2024 12:15 PM
Description
High sugar levels (hyperglycemia) in diabetes is known to cause deleterious effects on the eye and heart of individuals. This research studies (in vitro) the changes in both cell proliferation and oxidative stress on cultured retinal and cardiac cells. The cell lines studied were ARPE19 human retinal epithelial and H9c2(2-1) mouse embryonic cardiac fibroblasts. Both cell lines studied were exposed to a series of glucose levels (5.5mM, 17.5mM, 25nM, 33mM, 44mM, 50mM) as well as a series of lead levels (0.08µM, 0.4µM, 2µM, 10µM, 50µM) in a variety of media approved for cell culture maintenance. Extremely high levels of glucose are known to cause medical issues, especially in individuals exposed to normal levels of lead, for diabetics who tend to have a loss of retinal function, such as macular degeneration and damage to the heart by excessive proliferation of cardiac muscle fibroblasts thus causing hardening of the walls of the heart. This research has established the results of the toxic effects that normal concentrations of lead have in hyperglycemic conditions, and it will study the role of antioxidant compounds and their possible ability to reverse the toxic effects.
Cardiac/Diabetes Story
High sugar levels (hyperglycemia) in diabetes is known to cause deleterious effects on the eye and heart of individuals. This research studies (in vitro) the changes in both cell proliferation and oxidative stress on cultured retinal and cardiac cells. The cell lines studied were ARPE19 human retinal epithelial and H9c2(2-1) mouse embryonic cardiac fibroblasts. Both cell lines studied were exposed to a series of glucose levels (5.5mM, 17.5mM, 25nM, 33mM, 44mM, 50mM) as well as a series of lead levels (0.08µM, 0.4µM, 2µM, 10µM, 50µM) in a variety of media approved for cell culture maintenance. Extremely high levels of glucose are known to cause medical issues, especially in individuals exposed to normal levels of lead, for diabetics who tend to have a loss of retinal function, such as macular degeneration and damage to the heart by excessive proliferation of cardiac muscle fibroblasts thus causing hardening of the walls of the heart. This research has established the results of the toxic effects that normal concentrations of lead have in hyperglycemic conditions, and it will study the role of antioxidant compounds and their possible ability to reverse the toxic effects.