The Effect of EGCG on Arthrobacter sp. Phage Infection
Presentation Type
Poster
Faculty Advisor
Quinn Vega
Access Type
Event
Start Date
26-4-2024 2:15 PM
End Date
26-4-2024 3:15 PM
Description
The purpose of this study is to assess the impact of Epigallocatechin gallate (EGCG) on phage infection of EGCG treated Arthrobacter sp., a bacterial species found in different environments. Experiments were conducted to assess if EGCG treated Arthrobacter sp. will be susceptible to phage infection. Initially, bacterial viability was determined over time by measuring colony formation. Then, phage infection was monitored by infecting the bacteria with phage at different time intervals and observing plaque formation. Following this, various concentrations of EGCG were tested to determine a non-lethal concentration for Arthrobacter sp. Finally, the effect of EGCG on phage infection will be investigated by infecting EGCG-treated bacteria with phage and assessing infection rates. Each experiment was conducted multiple times. Through these experiments we hope to gain a better understanding of the mechanism behind ECGC’s antiviral properties. Through these studies, we were able to see a decrease in cell viability over time as well as a decrease in phage infectivity over time.
The Effect of EGCG on Arthrobacter sp. Phage Infection
The purpose of this study is to assess the impact of Epigallocatechin gallate (EGCG) on phage infection of EGCG treated Arthrobacter sp., a bacterial species found in different environments. Experiments were conducted to assess if EGCG treated Arthrobacter sp. will be susceptible to phage infection. Initially, bacterial viability was determined over time by measuring colony formation. Then, phage infection was monitored by infecting the bacteria with phage at different time intervals and observing plaque formation. Following this, various concentrations of EGCG were tested to determine a non-lethal concentration for Arthrobacter sp. Finally, the effect of EGCG on phage infection will be investigated by infecting EGCG-treated bacteria with phage and assessing infection rates. Each experiment was conducted multiple times. Through these experiments we hope to gain a better understanding of the mechanism behind ECGC’s antiviral properties. Through these studies, we were able to see a decrease in cell viability over time as well as a decrease in phage infectivity over time.