Start Date
17-10-2023 3:45 PM
End Date
17-10-2023 5:00 PM
Access Type
Open Access
Abstract
In collaboration with Prof. Mengyan Li at NJIT, we recently developed a rapid analytical method for per and polyfluoroalkyl substances (PFASs) using nano-electrospray high-resolution mass spectrometry (Nano-ESI-HRMS), which was recently published in Water Research, 2022, 219, 118542. This method was applied to aqueous film-forming foams (AFFFs) and wastewater samples collected from three local wastewater treatment plants (WWTPs). The method exhibited high sensitivity with lower limits of detection (LODs) of 3.2~36.2 ng/L for 22 target PFAS analytes. In AFFF formulations, Nano-ESI-HRMS enabled the first-time detection of trifluoromethanesulfonic acid (TFMS), perfluoroethyl cyclohexanesulfonate (PFECHS), 6:2 fluorotelomer sulfonyl amido sulfonic acid (6:2 FTSAS-SO2), N ammoniopropyl perfluoroalkanesulfonamidopropylsulfonate (N-AmP-FASAPS, n=3–6), ketone perfluorooctanesulfonic acid (Keto-PFOS), fluorotelomer unsaturated amide sulfonic acid (FTUAmS, n = 7), and 6:2 fluorotelomer amide (6:2 FTAm). Their structures were verified by the tandem MS analysis using collision-induced dissociation. Overall, Nano-ESI-HRMS enabled comprehensive PFAS quantitative analysis and suspect screening, applicable for rapid investigation and assessment of PFAS related exposure and treatment in environmental matrixes. In this talk, I will also introduce our recent effort in combining electrochemistry (EC) with mass spectrometry (MS) for protein absolute quantitation without using expensive standards. We expect it will make a good impact in quantitative proteomics.
Biography
Hao Chen graduated from Wuhan University in 1996 and obtained his Ph.D. degree from Purdue University in 2005. He is currently a professor in chemistry at New Jersey Institute of Technology (NJIT). Dr. Chen performs research on organic and biological mass spectrometry for bioanalysis and instrument development and has published 136 papers in peer reviewed journals.
Additional Links
ORCID
PFAS Screening and Protein Absolute Quantitation by Mass Spectrometry
In collaboration with Prof. Mengyan Li at NJIT, we recently developed a rapid analytical method for per and polyfluoroalkyl substances (PFASs) using nano-electrospray high-resolution mass spectrometry (Nano-ESI-HRMS), which was recently published in Water Research, 2022, 219, 118542. This method was applied to aqueous film-forming foams (AFFFs) and wastewater samples collected from three local wastewater treatment plants (WWTPs). The method exhibited high sensitivity with lower limits of detection (LODs) of 3.2~36.2 ng/L for 22 target PFAS analytes. In AFFF formulations, Nano-ESI-HRMS enabled the first-time detection of trifluoromethanesulfonic acid (TFMS), perfluoroethyl cyclohexanesulfonate (PFECHS), 6:2 fluorotelomer sulfonyl amido sulfonic acid (6:2 FTSAS-SO2), N ammoniopropyl perfluoroalkanesulfonamidopropylsulfonate (N-AmP-FASAPS, n=3–6), ketone perfluorooctanesulfonic acid (Keto-PFOS), fluorotelomer unsaturated amide sulfonic acid (FTUAmS, n = 7), and 6:2 fluorotelomer amide (6:2 FTAm). Their structures were verified by the tandem MS analysis using collision-induced dissociation. Overall, Nano-ESI-HRMS enabled comprehensive PFAS quantitative analysis and suspect screening, applicable for rapid investigation and assessment of PFAS related exposure and treatment in environmental matrixes. In this talk, I will also introduce our recent effort in combining electrochemistry (EC) with mass spectrometry (MS) for protein absolute quantitation without using expensive standards. We expect it will make a good impact in quantitative proteomics.